ldh activity assay kit  (Elabscience Biotechnology)

Journal: Materials Today Bio

Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair

doi: 10.1016/j.mtbio.2026.102783

Figure Lengend Snippet: PDA-HP hydrogel treated under NIR laser irradiation could improve the mitochondrial function. (A) ATP level assay of spinal cord tissue samples of various groups. The data of our experiments are reported as mean value ± SD ( n = 6, ns p = 0.6466, and ∗∗∗ p < 0.001). (B) MDA assay of spinal cord tissues in various groups. The data of our experiments are reported as mean value ± SD ( n = 6, ∗ p = 0.0427, and ∗∗∗ p < 0.001). (C) LDH assay of ND7/23 cells in various groups. The data of our experiments are reported as mean value ± SD ( n = 6, ∗ p = 0.0443, and ∗∗∗∗ p < 0.0001). (D) Results of MMP measured by JC-1. Scale bars are 50 μm. (E) The statistical chart of the ratio of JC-1aggregates to JC-1 monomers in various groups. The data of our experiments are reported as mean value ± SD ( n = 6, ∗∗∗ p = 0.0004, and ∗∗∗∗ p < 0.0001). (F) Immunofluorescence of ROS in various groups. Scale bars are 100 μm. (G) The statistical chart of DCF fluorescence. The data of our experiments are reported as mean value ± SD ( n = 6, ∗ p = 0.0182, and ∗∗∗∗ p < 0.0001).

Article Snippet: Cytotoxicity was evaluated using a commercial LDH assay kit (C0016, Beyotime, China) following the manufacturer's instructions.

Techniques: Irradiation, Multiple Displacement Amplification, Lactate Dehydrogenase Assay, Immunofluorescence, Fluorescence

Journal: Neural Regeneration Research

Article Title: Voltage-dependent anion channel 1 oligomerization regulates PANoptosis in retinal ischemia–reperfusion injury

doi: 10.4103/NRR.NRR-D-24-00674

Figure Lengend Snippet: PANoptosis and mitochondrial dysfunction in R28 retinal cells after OGD/R. (A) PI staining of R28 retinal cells subjected to OGD/R. A large number of PI-positive cells were observed in the OGD/R group. Scale bars: 40 μm. (B) Statistical analysis of PI-positive cells. (C) TUNEL staining of R28 retinal cells after OGD/R. There were very few TUNEL-positive cells in the CTL group, while a large number of TUNEL-positive cells were observed in the OGD/R group. Scale bars: 40 μm. (D) Statistical analysis of TUNEL-positive cells. (E) LDH release assay of R28 retinal cells subjected to OGD/R. (F) DCFH-DA probe detection of changes in intracellular ROS concentrations. There were very few cells exhibiting DCFH-DA fluorescence in the CTL group, whereas a large number of cells exhibiting DCFH-DA fluorescence were detected in the OGD/R group. Scale bars: 40 μm. (G) Statistical analysis of DCFH-DA fluorescence intensity. (H) Mito-tracker labeling showing mitochondrial morphology changes in R28 retinal cells subjected to OGD/R. The mitochondria in the CTL group mostly appeared as short rods, whereas in the OGD/R group, there were numerous mitochondrial fragments. Scale bars: 10 μm. (I) Mitochondrial membrane potential was detected by flow cytometry after JC-1 staining. (J) JC-1 assay of mitochondrial membrane potential after OGD/R treatment. Consistent with the flow cytometry results, we observed an increase in the number of green fluorescent cells in the OGD/R group compared to the CTL group. Scale bars: 80 μm. (K) Statistical analysis of JC-1 fluorescence intensity. Data are expressed as mean ± SD. All cell experiments included at least three independent replicates. * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed unpaired Student’s t -test). CTL: Control; DAPI: 4′,6-diamidino-2-phenylindole; DCFH-DA: 2ʹ,7ʹ-dichlorofluorescin diacetate; LDH: lactate dehydrogenase; OGD/R: oxygen-glucose deprivation/reperfusion; PI: propidium iodide; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

Article Snippet: A lactate dehydrogenase (LDH) cytotoxicity assay kit (Beyotime, Cat# C0016) was used to measure LDH release.

Techniques: Staining, TUNEL Assay, Lactate Dehydrogenase Assay, Fluorescence, Labeling, Membrane, Flow Cytometry, Two Tailed Test, Control, End Labeling